Requisition Form - UPMC Molecular Genomic Pathology Lab 2025

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As a subspecialty that involves the study of genetic and molecular changes in diseasesparticularly cancermolecular pathology is unique in combining pathology and molecular biology to study disease at a molecular level.
Adequate space, appropriate equipment and qualified personnel are required to establish a molecular pathology laboratory. One of the most important points that should be taken into consideration while designing a molecular pathology laboratory is to create a plan to prevent contamination.
Pathology requisition forms are used by physicians to test organ, tissue and bodily fluid samples for disease diagnosis. Other samples collected include blood, oral fluid, , cervical fluid, pleural fluid, pericardial fluid and ascetic fluid.
Molecular diagnostic laboratories should have at least two, preferably three rooms, each with a minimum area of 15 square meters, physically separated from each other to allow unidirectional workflow (from preamplification to postamplification) and preferably with separate ventilation systems.
Features of the three areas Area 1: Reagent preparation. This area is usually one of the cleanest areas in a molecular testing laboratory because it is typically free of biological material, such as DNA/RNA. Area 2: Sample preparation. Area 3: Amplification and post-amplification analysis.
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