ProStar 355 Refractive Index Detector Service Manual 2026

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An RI detector detects components based on the refraction of light in solution. As shown in Figure2, the flow cell of an RI detector is divided into the sample-side and reference-side cells. Prior to analysis, eluate is introduced into either cell, until the flow of the eluate becomes equilibrated.
The RI detector employs two beams of lighta reference beam and a sample beam. The reference beam flows through a cell filled with the pure mobile phase (devoid of analytes), while the sample beam passes through the flow cell containing the analyte-laden sample.
Refractive index (RI) detectors in high performance liquid chromatography (HPLC) are used to analyze samples that lack strong chromophores in ultraviolet (UV) or visible regions and are non-fluorescent.

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The refractive index of any other medium is defined relative to the refractive index of a vacuum, which is assigned a value of 1. Thus, a refractive index of 1.33 for water means that light travels 1.33 times faster in a vacuum than in water.
The light emitted from the lamp passes through the slit and transmits the flow cell as parallel beam. The transmitted light from the flow cell forms a slit image on the photodiode. The sample side is called the sample cell, through which the mobile phase containing the analyte passes continuously.
In summary, a refractive index detector functions by leveraging alterations in the refractive index of the mobile phase as analytes elute through a flow cell.

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