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We cant see bacteria because individually, they are too small to see with human eyes. We can, however, see great piles of them - we can grow bacteria on agar plates and generate millions to billions in a big pile called a colony that is quite visible to the naked eye.
i) The size of ZOI is influenced by the diffusibility of the chemical or antimicrobial agent. ii) The inoculums size has an impact on ZOI size. iii) The type of media used to cultivate the fungal or bacterial culture also affects the size of ZOI.
If a microbiology lab technician left the safranin out of the Gram stain procedure, what would be the result? Gram-positive cells would be purple, and Gram-negative cells would be colorless.
A Zone of Inhibition (ZOI) test is a quick way to determine the antibacterial efficacy of a treated product or surface.
What is ZOI? Zone of Inhibition is an assay that is conducted to test the efficacy of an antimicrobial agent in inhibiting the growth of specific bacteria. It refers to the clear area where there is no visible microbial growth which appears around the antimicrobial agents being tested on an agar plate.
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