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With the aggregated cells assay the NucleoCounter NC-200 counts nucleated cells by fluorescent staining of the DNA. Based on a carefully developed lysis buffer system, ChemoMetec A/S offers an optimized 2-step protocol for counting and determining viability of heavily agggregated cells.
How to use nc200?
0:32 1:54 Position the nc200 at the center of the forehead. Within five centimeters distance. The blueMorePosition the nc200 at the center of the forehead. Within five centimeters distance. The blue tracking light indicates the correct measurement. Area.
What is the NucleoCounter method?
The NucleoCounter YC-100 system allows reliable, fast and objective cell counting to be carried out based on an automated method of fluorescence microscopy. The system enables the user to stain and count cells with good protection against being exposed to potentially hazardous DNA staining dyes.
What is the range of NC 202?
Consistent Cell Counter Application type(s)Cell count viability analysis Optimal range 5104 to 1107 cells/ml Staining dye(s) Acridine orange (AO) and DAPI Excitation 2 LED light sources with peak wavelengths at 365 nm and 505 nm Emission (nm) A single dual-band emission filter: 410 460 nm and 540 650 nm13 more rows
Solution 17 contains two different dyes, staining the entire population of leukocytes and the non-viable leukocytes, respectively. After loading the Via1-Cassette it is placed in the NucleoCounter NC-200 where cell concentration and viability are determined.
What is the NC 200 method?
The NucleoCounter NC-200 offers a unique protocol to count and determine viability of cells grown on microcarriers without the need of detachment. With the reagents A100 and B, cells are lysed bringing the nuclei into suspension to be counted using the NC-200 (Figure 4.).
What is the optimal range for NC 200?
The working range of the NucleoCounter NC- 200 is 5x103 to 1x107 cells/ml in the tested solution, and the optimal range is 5x104 to 5x106 cells/ml.
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Non-Credit (NC)
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A prolonged period of storage or non-use will lower the voltage of the battery set (NC-200) due to self discharge. Always keep the battery set (NC-200) fully
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