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Gold: Contains a clot activator and special gel to separate serum from the cells. Purple: Contains a clot blocker and are used for complete blood count(CBC). Green Light Blue: Used for plasma testing. Plasma is left over after uncoagulated whole blood cells are removed by centrifugation.
Unlike red tubes, the type of blood produced in a green tube needed for testing is not serum but plasma. Green tubes are frequently used in requesting stat or immediate blood chemistry results for emergency cases. The grey tubes contain potassium oxalate that binds to calcium, thus, inhibiting coagulation.
The order of draw is an order of tubes phlebotomist should follow to prevent testing complications and cross-contamination of additives. The order of draw is Yellow, Light Blue, Red, Light Green, Dark Green, Lavender, and Gray. Way to remember this is by the saying Your brother Robert gives lovely gifts.
Withdraw the needle. Place gauze over the venipuncture site. For straight needles, withdraw the needle and activate the safety device. For butterfly needles, activate the safety device (push button on butterfly) while the needle is still in patients arm. Apply pressure to site until bleeding stops.
NOTE: All tubes sterile. Standard order of draw: BLOOD CULTURES, royal blue, red, light blue, SST (Gold), green, tan, yellow, pink, pearl, lavender.
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CBC - Lavender. Alkaline Phos - Green, SST, or Plain Red. WBC - Lavender. ALT (GPT) - Green, SST, or Plain Red. Hgb - Lavender. Amylase - Green, SST, or Plain Red. Hct - Lavender. Basic Metabolic Panel - Green, SST, or Plain Red. Tegretol (CRBAM) - Green, SST, or Plain Red. COAGULATION. PT INR - Blue.
Nowadays the order of blood draw established by the international guidelines is postulated to avoid cross-contamination between different additives contained in blood collection tubes, and it is as follows: blood culture bottle, non-additive tube, coagulation tube, clot activator, serum separator tube, sodium heparin,
Green top tube with sodium or lithium heparin: used for plasma or whole blood determinations. EDTA tubes: includes Lavender top, Pink top (used for blood bank testing), Tan top (used for lead testing), and Royal Blue top with EDTA (used for trace metal whole blood or plasma determinations).

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