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Dengue viruses are spread to people through the bite of an infected Aedes species (Ae. aegypti or Ae. albopictus) mosquito. Almost half of the worlds population, about 4 billion people, live in areas with a risk of dengue.
Causes: There are four different serotypes of the dengue virus: (DEN-1, DEN-2, DEN-3 and DEN-4). Getting infected with any of these types through a female Aedes aegypti mosquito causes dengue fever.
Interpretation of results A positive NS1 test result confirms dengue virus infection without providing serotype information. A negative NS1 test result does not rule out infection. People with negative NS1 results should be tested for the presence of dengue IgM antibodies to determine possible recent dengue exposure.
About 1 in 20 people who get sick with dengue will develop severe dengue. Severe dengue can result in shock, internal bleeding, and even death. If you have had dengue in the past, you are more likely to develop severe dengue. Infants and pregnant women are at higher risk for developing severe dengue.
Dengue fever is transmitted from one person to another through the bite of an Aedes aegypti mosquito. A healthy mosquito becomes infected when feeding on the blood of a person infected with the virus, to then transmit it to healthy people when it moves to feed on their blood.
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Serology for dengue virus-specific IgG and IgM antibodies can help confirm a primary or secondary diagnosis. On average, 5 days after infection, both main and secondary infections produce IgM, whereas IgG is produced 2-4 weeks after primary infection and practically immediately after secondary infection.
For patients presenting during the first week after fever onset, diagnostic testing should include a test for dengue virus (rRT-PCR or NS1) and IgM. For patients presenting 1 week after fever onset, IgM detection is most useful, although NS1 has been reported positive up to 12 days after fever onset (Figure 3-01).
Key facts. Dengue is a viral infection caused by the dengue virus (DENV), transmitted to humans through the bite of infected mosquitoes. About half of the worlds population is now at risk of dengue with an estimated 100400 million infections occurring each year.

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