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Well-made peripheral smears can be prepared by starting with only a drop of blood at one end of a clean glass slide. The drop is smeared lightly and quickly with a wedge technique so as to leave a thin feather edge where all cells may be examined individually, particularly red blood cells.
The peripheral blood film (PBF) is a laboratory work-up that involves cytology of peripheral blood cells smeared on a slide. As basic as it is, PBF is invaluable in the characterization of various clinical diseases.
The Ideal Blood Smear An ideal slide is neither too thin nor too thick. It should end about two-thirds to three-fourths of the way down the slide. The end of the smear should be thin enough that it has a rainbow-like reflectiveness about it, and there should be no streaks at the very edge.
Peripheral Blood Smears (PBS)
Guideline normal values: 1.3-3.5 x 109/L, comprising 20-45% of WBCs.
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Healthcare providers use peripheral blood smear tests to diagnose blood disorders, blood cancers and infections. These conditions can happen when bone marrow cells mutate and become abnormal cancerous cells known as blasts. Examples include: Leukemia: This is a cancer of your blood and bone marrow.
A well-made peripheral smear is thick (red blood cells are overlapping) at the frosted end and becomes progressively thinner with good separation of cells toward the opposite end. The so-called zone of morphology, the area of optimal thickness for light microscopic examination, should be at least 2 cm in length.

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