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In general, DV200 score above 30% is considered a good RNA quality sample Visium CytAssist Recommendations and Illumina Tech Note on evaluating FFPE sample quality).
What is a DV200 value?
*The DV200 is the percentage of RNA fragments 200 nucleotides. Although RIN values for these samples lie within a relatively narrow range (2.33.2), the size distribution of the RNA varies greatly among the samples.
What does DV200 mean?
Among methods for evaluating RNA quality, the RNA integrity number equivalent (RINe) is widely used, whereas the DV200, which evaluates the percentage of fragments of 200 nucleotides, is also used as a quality assessment standard.
How to interpret DV200?
DV200 is the percentage of RNA fragments 200 nucleotides. It is important to note that while DV200 are predictive of the average RNA quality of the section surveyed, DV200 scores may vary within a block. For example, the outer layer may be exposed to more environmental factors.
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