The new DUBscan Kit Application Note. - Ubiquigent 2025

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The document is an application note for the DUBscan™ Kit, designed to identify deubiquitylating enzymes (DUBs) that facilitate the deubiquitylation of substrate proteins. It details the kit's components, including a 96-well plate with 47 DUBs and control reagents for conducting deubiquitylation reactions. The document outlines various experimental applications, such as analyzing in vitro and in vivo ubiquitylated substrates, exploring DUB specificity, and validating enzyme activity. It also presents example experiments demonstrating the kit's utility in studying K63-linked ubiquitin chains and cellular substrates like IRAK1.

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