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Glucose Clamp Forms core 2008 rtf - med upenn

Glucose Clamp Forms core 2008 rtf - med upenn-2025

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The document is a submission form for a glucose clamp study conducted by the Mouse Phenotyping, Physiology and Metabolism Core. It requires information about the principal investigator, study details, and mouse specifications. The form outlines different pricing options for glucose clamp procedures based on the use of tracers and includes sections for recording mouse data and charges.

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What is the pancreatic clamp technique?

To perform a hypoglycemic clamp individualized intravenous insulin and glucose infusions are titrated to achieve and maintain a pre-defined blood glucose target for a specified period of time, e.g. 30-60 min, at euglycemic and then hypoglycemic target levels.

What is the glucose clamp procedure?

Glucose clamp techniques make use of intravenous infusions of glucose to raise blood glucose levels 6.9 mmol/L above basal levels. Constant infusion adjustments maintain the basal level and are followed by arginine stimulation to measure total insulin secretory capacity.

What is a hyperglycemic glucose clamp?

For the hyperglycemic clamp, 30% glucose is infused into the vein, and blood glucose levels are measured from the arterial blood every 5 min or 10 min. The infusion rate of 30% glucose is increased until the blood glucose level becomes 250 mg/dL. Blood is collected to measure insulin concentrations.

What is the glucose infusion rate?

A pancreatic clamp is a technique in which we temporarily shut off the bodys production of insulin with intravenous (into the vein) hormone mixtures that allow researchers to vary levels of insulin, over about 7 hours.

What is the glucose infusion rate clamp?

The glucose clamp technique was originally developed based on steady-state intravenous insulin infusion and adjustment of the glucose infusion rate to maintain a constant glucose concentration. The components required include a method to take blood glucose samples, a blood glucose analyzer, and a glucose infusion pump.