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Evaluation of a Test Article in the Salmonella typhimurium - DTIC - dtic

Evaluation of a Test Article in the Salmonella typhimurium - DTIC - dtic 2025

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The document is a final report from SITEK Research Laboratories detailing the evaluation of Ethylenediamine dinitrate (EDDN) for its mutagenic potential using the Salmonella typhimurium/Escherichia coli Plate Incorporation Mutation Assay. The study, conducted under Good Laboratory Practice guidelines, tested EDDN at various concentrations with and without metabolic activation from induced rat liver S-9. Results indicated that EDDN was not mutagenic in most strains but showed weak positive results in strain TA100 at the highest concentration tested. The report includes detailed methodologies, results, and compliance statements.

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What is the Ames test a method for detecting?

Ames test is a method for evaluating mutagenic effects of implant device, chemicals, and drug utilizing bacteria to detect carcinogens and mutagens.

What is Salmonella typhimurium reverse mutation assay?

(1) A reverse mutation assay in Salmonella typhimurium detects mutation in a gene of a histidine requiring strain to produce a histidine independent strain of this organism. (2) Base pair mutagens are agents which cause a base change in the DNA.

What is the Ames test Salmonella typhimurium?

Ames test devised by a scientist Bruce Ames is used to assess the potential carcinogenic effect of chemicals by using the bacterial strain Salmonella typhimurium. This strain is mutant for the biosynthesis of histidine amino acid. As a result they are unable to grow and form colonies in a medium lacking histidine.

What does the Ames test tell you?

The Ames test is a commonly used method that utilizes bacteria to test whether a particular chemical can cause mutations in the DNA of the test organism. It is a biological assay that is formally used to assess the mutagenic potential of chemical compounds.