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RNA probes are stretches of single-stranded RNA used to detect the presence of complementary nucleic acid sequences (target sequences) by hybridization. RNA probes are usually labeled, for example with radioisotopes, epitopes, biotin or fluorophores to enable their detection.
DNA and RNA labeling techniques. Oligonucleotides can be labeled at either the 3' or the 5' end. Using polynucleotide kinase and ATP-gamma-32P, the 5' end is labeled. Using terminal transferase and deoxynucleotide triphosphate labeled on the alpha phosphate, the 3' end is labeled.
Abstract. Radioactive end-labeling is useful for visualizing and allowing the detection of nucleic acids at trace concentrations. Radioactive end-labeling can be carried out on RNA, DNA, or other modified nucleic acids.
Nucleic acids are readily labeled with tags that facilitate detection or purification. A variety of enzymatic or chemical methods are available to generate nucleic acids labeled with radioactive phosphates, fluorophores, or nucleotides modified with biotin or digoxygenin for example.
A polymerase ribozyme can be used to label the 3\u2032 end of RNA or DNA molecules by incorporating a variety of functionalized nucleotide analogs.

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Radioactive end-labeling is useful for visualizing and allowing the detection of nucleic acids at trace concentrations. Radioactive end-labeling can be carried out on RNA, DNA, or other modified nucleic acids. For RNA, the uses of end-labeling extend beyond simple detection of the intact RNA.
Nucleic acids are readily labeled with tags that facilitate detection or purification. A variety of enzymatic or chemical methods are available to generate nucleic acids labeled with radioactive phosphates, fluorophores, or nucleotides modified with biotin or digoxygenin for example.
Product Listing Application Overview. Detection of specific nucleic acid sequences can be accomplished by hybridization with a labeled RNA probe. RNA probes are sequences of variable lengths that are used to detect the presence of complementary nucleotide sequences in a sample.
Labeling of DNA probes for in situ hybridization is most often performed by enzymatic incorporation of labeled dNTPs using Taq DNA polymerase (= Polymerase Chain Reaction (PCR)) or a mixture of DNase I/ DNA polymerase I (=Nick Translation) (Tab. 1) [1,2].
There are two ways to label a DNA molecular; by the ends or all along the molecule. End labeling can be performed at the 3'- or 5'-end. Labeling at the 3' end is performed by filling 3'-end recessed ends with a mixture or labeled and unlabeled dNTPs using Klenow or T4 DNA polymerases.

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