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This tutorial discusses capture sequencing and its application in enriching for target transcripts, specifically voltage-gated calcium channels. Enrichment is necessary due to low RNA expression levels, particularly in scarce source material like human brains. Two main methods of enrichment, PCR targeted sequencing and capture sequencing, are compared. PCR targeted sequencing amplifies the target before sequencing, providing high specificity and depth of reading. Capture sequencing allows for detection of rare isoforms and multiplexing of samples, with recent advancements in barcoding technology increasing potential for scalability.
