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thank you so im gonna be talking about capture sequencing and what were doing with it um so to start with why are we enriching for our um target transcripts um thats because were interested in voltage-gated calcium channels as potential drug targets but they have very low rna expression levels so if we didnt enrich wed have very few reads of interest which is a particular problem when our source material human brains is quite a scarce resource so weve been using two main methods to enrich pcr targeted sequencing and capture sequencing the pcr targeted sequencing uses a specific primer pair to amplify the target before sequencing and that makes it really really specific and you get really high depth of reading per your target which means you can detect really rare isoforms and you can also multiplex many samples which was limited by the number of barcodes but nanoport announced yesterday theyre making more barcodes so thats not true anymore but you wont amplify transcripts th
