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foreign [Music] once again today were going to be talking about super resolution microscopy systems and for those of you who may not work a lot with microscopy or may not work particularly with super resolution that simply means being able to see fine structures that otherwise laser scanning microscopy with light wouldnt be able to Define so this is an example image of comparing a wide field microscope where our illumination goes across the entire plane on a structure called vimentin here is the same region where we do a confocal scan we clean up a lot of these fibers and structures but we still have quite a bit of blurriness that hopefully what you can tell on your screen may be better than a projector is that theres a fine mesh of these structures here that are able that were able to actually resolve through super resolution Technologies and particularly with todays Focus were going to be discussing super resolution related to fluorescence microscopy fluorescence as a physical