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[Music] with gloved hands and safety glasses open the gauze package gloves must be changed after obtaining each sample to avoid cross-contamination of any subsequent samples the method requires gauze to be moistened with 2 milliliters of hexane using the ampules provided use one ampule of hexane on one gauze pad per sample carefully break the elongated end of the ampule while holding it over the gauze moisten the gauze pad with all the contents of the ampule properly disposed of empty ampules and elongated ends using only one side of the wipe thoroughly wipe a designated area specified using repeated horizontal and vertical motions wipe an area about 100 square centimeters rubbing the entire area side to side then up and down wipes may also be used on irregular surfaces where it may not be possible to wipe 100 square centimetres after you have completed the sample collection fold the wipe sample soiled side facing in and place it in the supplied collection vial and cap the vial identi