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okay okay no problem okay so today im going to talk about our recent work on a computer algorithm that overcomes the revolution limits as alexandra as landrieu i have intro introduced uh my lab have been being done a lot of things but the main thing is we want to do imaging in live cells in lives conditions so before i introduce our recent work i need to go back a little bit to talk about the ultra-sensitive hyacinth structure illumination microscope we did five years ago so you guys must um i i would guess this is a imaging forum so many of you guys knows the structured illumination microscope so i will not talk about the basic theory i just talked about how it did so when you are doing a two-dimensional structuring motion microscopy you acquire 9 images with this kind of a three pattern angle like this stripe python going up and down and 120 degrees and 240 degrees okay at each angle you have three different faces and after you capture uh nine images you do a fourier transformation