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[Music] hello and welcome to explore bio in todays video i will explain what are esd uni gene transcript and differences among them at last i will also highlight how these are related to reads contigs and scaffold that you have learned in my previous video its link is given in the description below if you have watched my genome story video i have explained that not all genes are expressed at all the times organisms turn on and off their genes in response to environmental and developmental cues expression of one or more genes provide critical biological information such as health status or disease there are techniques such as microarray transcriptome sequencing and others that provide information about the gene expression so the three terms that i am going to discuss today represents the expressed part of the genome the first is esds ests or express sequence tags are short dna sequences of usually less than thousand bases long obtained by single pass sequencing of complementary dna fr