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hello everyone and welcome to our video on quantifying mmp activity with image j and normalizing samples to an mmp standard the goal of this segment of our series is to show you how to take a stained symmogram like this one and quantify the bands of mmp activity such that you can graph it as shown here in order to do this procedure youre going to need some basic software microsoft office is what i use but similar programs to powerpoint and excel will also work i also use a free program called imagej to quantify the mmp activity bands and you can download that at this link and youre also going to need the scanned images of your xymograms before and after you stain them and they should be good quality images like around 300 dots per inch in the last video we showed you how to use the molecular weight protein ladder in order to identify different mmps in your zymograms now the next step is for us to quantify the bands of mmp activity and image j so when you first open up image j this i
