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hi Im John Griffin microscopy imaging officer at the Australian cancer research foundations cancer biology imaging facility at the Institute for Molecular bioscience on the University of Queenslands st. Lucia campus welcome back to our tutorial series on microscopy and image processing in this installment well be looking at image stacks and hyper stacks an image chain a basic photo micrograph represents a change in some physical property across two dimensions for optical microscopy this property is generally something like brightness or optical density many microscopes also use grayscale cameras or other detectors that dont collect the color of light falling on them thus we get a flat image with one color the objects were studying are often more complex than this so how do we record and represent other kinds of information like 3d structure changes over time or information on different colors the answer is we use an image stack multiple images displayed sequentially in the same wi
