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hi im jennifer waters the director of the nikon imaging center at harvard medical school and this micro course is on the point spread function the point spread function is a fundamental principle in light microscopy and many of the concepts that we would like to understand about microscopy both on a theoretical and a practical level require an understanding of the point spread function were going to focus our discussion of the point spread function on resolution the formal definition of resolution is the ability to distinguish objects that are separate from one another in reality in the sample as separate from one another in the image of the sample that is generated by the microscope these are dic images of natures perfect test specimen diatoms diatoms have pores in their silica shells that are very regularly spaced and in some species the spacing between the pores is right at the resolution limit of the microscope both of these images were taken using the same magnification but th