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in this video demonstration we will see about the most commonly used the blood smear staining technique for the microscopic examination the leashman staining this protocol is also a differential staining method which is used to variably stain and differentiate the various cells of the blood as well as to identify the microorganisms present in the blood first we will see about the blood collection generally for hematology the whole blood from the patient is collected in an edt a coated vacuum container the vacutainer the edta the ethylene diamine tetracetic acid which is an anticoagulant used to prevent clotting of blood now we will see the procedure for the preparation of blood smear the most commonly followed technique for blood smear preparation is the wedge or the push technique take a clean and grease free microscopic glass slide with the help of micropipette or syringe needle place a small drop of blood exactly at the midline at the end of glass slide take another glass slide pla