Slide stain in rtf smoothly

Aug 6th, 2022
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How to slide stain in rtf quicker

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How to Slide stain in rtf

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good afternoon today Im going to be teaching you how to stain a slide the first thing that you want to do is you want to obtain a clean microscope slide the second step that youre going to do is youre going to place your specimen in the center of the slide today what were going to be doing is were going to be making onion cell slides and staining the cells and onion so what I want to do is get a very thin piece of onion skin and once I get that thin piece of onion skin I want to place it onto my slide and try to get it on there as flat as I possibly can all right our next step after we get our specimen placed on the slide is to add a couple of drops of stain the stain that Im using today is called methylene blue and what its going to do is its going to stain all the organelles in the onion skin here Im going to place a couple of drops onto my onion skin all right my next step after I place that stain as I need to put a coverslip on top of my slide now this is a tricky part be

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Eosin Y. Eosin Y Stain is a reversible, fluorescent red, acidic dye, commonly used in hospital histology labs. Eosins most important medical uses are in blood and bone-marrow testing, including the PAP smear. It can also test for protein in plant, animal and blood specimens.
Iodine Staining In addition to making slides easier to examine, iodine is often used as a starch indicator in science experiments. When iodine is introduced to a substance that contains starch, it will turn to a dark blue or blue-black hue.
The most basic reason that cells are stained is to enhance visualization of the cell or certain cellular components under a microscope. Cells may also be stained to highlight metabolic processes or to differentiate between live and dead cells in a sample.
There are several types of stains you can use at home including: food coloring, iodine, malachite green (ick fish cure), and methylene blue. You can purchase food coloring at a grocery store, iodine at a pharmacy, and the malachite green and methylene blue can be purchased at an aquarium store.
The staining procedure: Make sure the sample on the slide is dry. Dip the slide in each jar between six-10 times (10-15 seconds in each solution). Wash the slide with water after jar number 3 ONLY. Dip the slide in the water jar. Never wash the slide in between the staining process. Alternative to Jar 4:
There are several types of stains you can use at home including: food coloring, iodine, malachite green (ick fish cure), and methylene blue. You can purchase food coloring at a grocery store, iodine at a pharmacy, and the malachite green and methylene blue can be purchased at an aquarium store.
The Most Common Stains Used Haematoxylin and eosin combined make up a common stain most histology labs use first. No doubt youre familiar with at least haematoxylin, which is sometimes used alone. Combining this with eosin helps bring better definition to tissues and cells using a specific color chart.
Hematoxylin is a basic dye that is commonly used in this process and stains the nuclei giving it a bluish color while eosin (another stain dye used in histology) stains the cells nucleus giving it a pinkish stain (Victor, 2013).

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