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nto the stain so that the edge of the coverslip is submerged first. Allow the coverslip to sit in the stain for a few minutes, then gently lift it out using the tweezers. Place the coverslip onto a clean glass slide and remove any excess stain by gently blotting with a tissue. The slide can now be observed under a microscope, utilizing various magnifications to examine the fungus for its structure and spore production. This staining technique helps to highlight the different components of the fungus, making it easier to identify and study. Overall, the process involves transferring a block of agar from a known culture, growing it on a fresh agar plate, staining a coverslip with lacto phenol cotton blue, and then examining the stained coverslip under a microscope.
