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[Music] so welcome once again to staining protocol and this time were comparing two types of trichrome the masses trichrome and the MSB steam so now all of these four slides that you can see here they originally stained with the voids hematoxylin just as we normally do for Van Giessen stain as well so theyve obviously already been taken down to water ordinarily wed also employed post fixation in picric acid but just for simplicity were going to focus here on the more difficult elements of each of these stains okay so all four of these slides have been stained in the vigor its hematoxylin for 10 minutes thats just the standard time weve stained upside down as you can see just to limit the amount of stain precipitate onto the slide now differentiation keep this really brief I normally just go in out straight back for the water bath but Im just going to jiggle it a little bit just to ensure that at least a little bit of mixing on the surface of the slide that might actually be ove