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this demonstration is to show you how to perform a simple stain before staining a slide you need to heat fix the slide place the slide on the staining rack which is over top of the tray a simple stain requires only one reagent crystal violet youre going to add several drops of the crystal violet onto the slide and make sure the smear is covered its okay that you touch the dropper to the slide because the bacteria have been killed when you heat fixed allow the stain to remain on the slide for 30 seconds after 30 seconds tilt the slide over the tray using the large bottle of deionized water in the squirt bottle you want to rinse the slide place the slide on a piece of paper toweling get a piece of Bibulus paper fold it over once twice and you gently want to block the slide dry after doing a simple stain you should see the purple color of the stain on the slide youre now ready to focus the slide under the microscope and remember when you focus a stain slide you always start with scan
