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How many replicates are needed for DESeq2?

At least six replicates per condition for all experiments. At least 12 replicates per condition for experiments where identifying the majority of all DE genes is important. For experiments with

What is the minimum number of replicates for statistical analysis?

Biological replicates are required if inference on the population is to be made, with three biological replicates being the minimum for any inferential analysis.

What does 3 replicates mean?

verb. To make three copies of. adjective. Having three copies; triple; threefold. Supplement.

Is repetition and replication the same?

3:34 8:47 Repetition vs Replication - YouTube YouTube Start of suggested clip End of suggested clip Often do with their own experiment. They conduct multiple trials they do their experiment. MultipleMoreOften do with their own experiment. They conduct multiple trials they do their experiment. Multiple times of the same procedure. In order to see if they can repeat their same results over and over

How to run deseq2 without replicates?

For an experiment without replicates, you should just run DESeq() as normal.

What is the difference between repeat and replicate?

Repeat and replicate measurements are both multiple response measurements taken at the same combination of factor settings; but repeat measurements are taken during the same experimental run or consecutive runs, while replicate measurements are taken during identical but different experimental runs, which are often

How many cells needed for bulk RNA-seq?

Start with just 100 cells Because we use single-cell reagents, we can perform a bulk RNA sequencing analysis on extremely low sample volumes. We only need 100 cells for bulk RNA sequencing.

What is the difference between replication and repeated measurement?

Repeated measures involves measuring the same cases multiple times. So, if you measured the chips, then did something to them, then measured them again, etc it would be repeated measures. Replication involves running the same study on different subjects but identical conditions.

Can I use FPKM for DESeq2?

Data measured by FPKM expression units is not suitable for differential expression comparisons. if you align your data with STAR, just use featureCounts to derive raw counts, which you will then use as input to DESeq2.

Does DESeq require replicates?

You need to have biological replicates of each experiment condition in order to estimate dispersion properly. If there are no replicates, DESeq will estimate dispersion using the samples from the different conditions as replicates.

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