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DNA is a double helix made up of two twisted strands containing four chemical bases represented by the letters A, C, G, and T. The strands are complementary, with specific base pairing rules. DNA replication begins with the separation of the two strands by an enzyme called helicase, creating a replication fork. A primer, made by an enzyme called primase, marks the starting point for DNA replication. DNA polymerase binds to the primer and builds a new strand of DNA using the original DNA strand as a template.