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[Music] [Laughter] hi and welcome to the channel which covers mainly physiology pharmacology and image processing i thought what i wanted to do today was just a quick video on background subtraction and three ways in which you could subtract the background from an image this is related to the previous video that i did in which we used binary masks in order to extract information from fairly noisy images but lets say you just had a kind of a noisy background and you wanted to subtract that background noise im using image j and the image im using here is a few cells these are i think these were alien cap cells they have a fluorescent drug on them quite a low concentration of the drug so theres not a great deal of fluorescence let me just make this a greyscale lookup table so you can see a little bit better okay not too bad but lets use the 323 rgb lookup table if i zoom in in the background here uh you can see its its a fairly noisy background and if you look at the values up her