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mRNA and proteins are produced as needed in organisms. Some genes are constantly expressed, undergoing constitutive transcription, while others are only produced at specific times in certain cells through regulated transcription. Transcription is regulated by proteins called transcription factors, which interact with specific genes to activate transcription. Most eukaryotic species have over 1000 transcription factors. Regulated transcription starts when a signal is received by the cell, initiating a signal transduction cascade.