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In this video tutorial, the focus is on preparing an uncontaminated bacterial culture using aseptic technique and investigating the impact of antibiotics on bacterial growth. Bacteria reproduce through binary fission and can double every 20 minutes under optimal conditions. Bacterial cultures can be grown in nutrient broth solutions or agar gel plates, which provide the necessary nutrients for growth and division. Agar gel plates solidify the nutrient broth with agar, allowing for the growth of bacterial colonies. Triple biology students will benefit from this guide on culturing bacteria effectively.