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hello everyone in this video I will show you how the data must be placed on Origin pro 8.5 to plot this bar chart with multiples columns and error bars before plotting you have to allocate your data in this way on the sheet lets take a closer look at this first the labels of the independent variables independent variables must be placed on the X columns and each independent variable must be allocated on a row then they will appear this way which corresponds to this now the dependent variables pendant variable must be add-on separated column which has to be designated as Y column which corresponds to this the error bar can be the value for the standard deviation or the mean error or both they work as the dependent variable but they must be designated as Y error which corresponds to this now you just have to select your whole she go to plot call bar pie then click on column or you can go to plot column bar pie and then click on bar and now you just need to edit the graph and youre don