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every one professor Davis here from chem survival comm and the YouTube channel chem survival and today I want to talk to you a little bit about packing and running chromatography columns since were coming up on that in my course I thought you might be coming up on it and yours and you like a little bit of advice so the goal of running a chromatography experiment is always to resolve two or more compounds in space a separation of two or more compounds means physically getting them in two different regions of space where we can collect them now this depends on a number of factors whether or not well be successful not the least of which is the quality of our column comes must be of uniform length diameter and density in order to function optimally and well take a look at why this is in just a little bit additionally the analyte solution that we load needs to be not only very concentrated but loaded in a fairly narrow band to facilitate or minimize the amount of distance that means to