Operate formula resolution easily

Aug 6th, 2022
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How to operate formula resolution

5 out of 5
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[Music] go really quickly over the axial resolution formula I want to go over this specifically because Ive had two or three questions in the past few days over this very specific formula and as I was talking to other students I realized that theres one little thing that trips people up and makes you assume the relationships are one way were they actually are another way so if we quickly look at what the formula is you can see here we have our axial resolution is equal to one half of the spatial pole sling generally I teach you to write your formulas in the way I have here in the Triangle if you have one thing multiplying another you write the fraction basically whatevers on top goes in the top of the pyramid whatevers on the bottom goes on the bottom of the pyramid and then whatever that fraction equals will go on the other side of the bottom of the pyramid so you can see that our axial resolution will equal our spatial pole plane over two which is the same as saying one-half of

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Microscope Resolution Equation Here is one equation we use: (r) = 0.61 / NA, where r is resolution, is the imaging wavelength (550nm on average), and NA is the numeric aperture of the objective lens in question.
d = /(2NA) where is the wavelength of light used to image a specimen. If using a green light of 514 nm and an oil-immersion objective with an NA of 1.45, then the (theoretical) limit of resolution will be 177 nm.
Since optical resolution (R) is a function of the wavelength () of the light and the numerical aperture (NA) of the lens (R=(f) /2NA), both the lens system and the light filtering system are important in WSI.
Definition 2: Resolution can be expressed as the total number of pixels. With megapixel cameras, the resolution is generally the total number of pixels, divided by 1,000,000, and rounded off.

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