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alright thanks so much for for inviting me and for letting me talk about some work that I did with my former students Rob Pedro and keep doogal dont worry my talks only 45 minutes long so well have any time for the break okay all right so we all know that the amount of genomic data that is available is a huge and increasing really rapidly and so just looking at one public database the NIH sequencing read archive it has over three pedda basis of publicly available sequencing data comprising short read sequent experiments from you know whole genome sequencing efforts metagenomic sequencing efforts and many thousands in fact over 150,000 gene expression experiments from art using RNA seek and the goal of or one of the goals of my group is to develop computational techniques that enable fast discovery from data at this scale and in particular for this talk I want to talk about these gene expression experiments and the challenge of estimating gene expression abundance from RNA seek data