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hello welcome to watch the creative proteomics videos about proteomics quantification today we are going to learn some basic knowledge about label-free quantitation methods label-free protein quantification is a mass spectrometry based method for identifying and quantifying relative changes in two or more biological samples instead of using a stable isotope containing compound to label proteins there is no limit on the number of samples and label-free protein quantification and its in principle applicable to any kind of sample including materials that cannot be directly metabolically labeled it is ideal for large sample analyzes and clinical screening or biomarker discovery experiments in general label-free approaches can be divided into two distinct groups according to the method used for data extraction the quantification can be inferred by counting the number of peptides or spectra assigned to a given protein and therefore are generically called spectral counting methods on the ot