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Hi everyone. Im Tristan, and Im very proud to introduce my new baby ISOLDE. The name stands for Interactive Structure Optimisation by Local Direct Exploration, but the simplest description is that its a new interactive model building environment. In particular, its designed to ease the often fraught task of building and refining models into low-resolution maps. So before we get properly started, Id like to spend a minute or two discussing why thats important. Theres quite a lot going on in this plot, so let me break it down for you. On the y axis we have the MolProbity score which combines common measures of structural error, namely atomic overlaps or clashes, unlikely backbone conformations and unlikely sidechain conformations into a single log-scale score, where lower is better. On the x-axis we have the R-free, a measure of how well the model fits the data. Again, lower is better. So the bottom left corner is where we want to be. Each spot represents one three point five to
