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to calculate by also index you first use a disclosing agent become in liquids that you can paint on or tablets that the patient can do you then count the sites of biofilm that are on the gingival third of the tooth on the buccal and lingual of each tooth to calculate the amount of biofilm you then total a number of biofilm sites then calculate the number of T through the patient has for example our patient has 28 teeth you then times the number of teeth by two to account for buccal and lingual to surfaces you then divide the number of biofilm sites by the surface in this case it would be point two nine which you would turn into a percentage so that patient has 29 percent of their teeth covered in biofilm