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in this tutorial I will show you how to perform global fitting with the dose response function dose response analysis is used to compare critical parameter values between different compounds such as the EC 50 or ic50 parameter origin provides built-in dose response fitting functions and in this tutorial I will also show you how to compute EC 50 or ic50 using what is called derived parameters the data from a fictitious assay is substrate titration for an enzyme in the presence of a competitive inhibitor at different concentrations lets go ahead and create a scatter graph notice the legend is the same for all the curves so what Im going to do is change this in fact before I do that I will make the y-axis title to be velocity which was in the legend and then we will pick up the information here for the legend graph update legend and then lets use the long name and units so that information was picked up from here and displayed in the legend lets go ahead and bring up the curve fitter
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