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foreign here is an image and we will use image J software to count the number of holes and measure their areas open the image of Interest using image J software the first step is to change the image type into an 8-bit image change the brightness and the contrast of the image if required before changing the image type next adjust the threshold by selecting image edges and threshold adjust the threshold such that only the holes are completely red in color now in the threshold window click on apply if there are gaps within the holes these gaps can be filled in the next step to fill the gaps click process binary and fill holes the gaps cease to exist in case the threshold step leads to holes connecting with one another it may need a separation step it can be performed by clicking on process binary and erode the holes are now well separated from one another now click on analyze and set measurements in the set measurements window select all the parameters required to perform the analysis suc