Extend columns resolution easily

Aug 6th, 2022
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How to extend columns resolution

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in this video we will see how to extend the column width so that it fits the width of the data contained in them and also how to extend the rows height so that it fits the data containing them as well and i have an example right here and if we look closely we can see that we have some cells that have some data that is bigger than the column width as well we have some rows that have multiple lines of text and this is as well bigger than the overall rows height and let me show you how to extend everything so that it looks nice and professional so first im going to select all the data that i have im holding ctrl and then i press a and just like that all the data has been selected and then i go to home and in home i identify the sales group over here and i click on format and first were going to start with the row height we have this option right here auto fit row height im selecting it and just like that you see the rows that had multiple lines of data have now been extended to fit t

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Column length and efficiency If the column length is reduced, the number of theoretical plates in the column will be correspondingly reduced and therefore the resolution of the HPLC system.
Chromatographic parameters The advantage of switching to longer columns is that the theoretical plates will increase and so will the resolution. The number of theoretical plates (N) is directly proportional to the length of the column and inversely proportional to the particle diameter2.
A longer column generally improves the separation. The trade-off is that the retention time increases proportionally to the column length and a docHub peak broadening will be observed as well because of increased longitudinal diffusion inside the column.
Chromatographic parameters The advantage of switching to longer columns is that the theoretical plates will increase and so will the resolution. The number of theoretical plates (N) is directly proportional to the length of the column and inversely proportional to the particle diameter2.
How to Improve Resolution in HPLC Increasing column length. Decreasing particle size. Reducing peak tailing. Increasing temperature. Reducing system extra-column volume.
HPLC resolution R Resolution is measured by dividing the difference in peak retention times by the average peak width. Resolution can also be expressed in the Resolution Equation as a combination of the factors (separation, efficiency, and retention) that affect this value.
HPLC Instrument Considerations Standard columns for reversed phase, normal phase, and ion exchange chromatography typically range from 3.9 to 4.6 mm internal diameter and 15, 25, and 30 cm in length. Standard columns for gel permeation chromatography (GPC) are 7.8 mm by 30 cm.
On the first column click A to select the column, and then right click and select Column Width. Type the width that you want for your column. Click OK.

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