Extend columns certificate easily

Aug 6th, 2022
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How to extend columns certificate

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in this video we will see how to extend the column width so that it fits the width of the data contained in them and also how to extend the rows height so that it fits the data containing them as well and i have an example right here and if we look closely we can see that we have some cells that have some data that is bigger than the column width as well we have some rows that have multiple lines of text and this is as well bigger than the overall rows height and let me show you how to extend everything so that it looks nice and professional so first im going to select all the data that i have im holding ctrl and then i press a and just like that all the data has been selected and then i go to home and in home i identify the sales group over here and i click on format and first were going to start with the row height we have this option right here auto fit row height im selecting it and just like that you see the rows that had multiple lines of data have now been extended to fit t

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AB: A LC column can be fine for a long period of time and has no defined shelf life, but it is dependent on what storage solvent was used for column storage. If the column was properly flushed after use and stored in 100% organic solvent such as acetonitrile, the column will survive for many years.
By adding a guard column, we were able to extend the life of our GPC columns from 6 to 24 months without compromising chromatographic performance. By adding a guard column, we were able to extend the life of our GPC columns from 6 to 24 months without compromising chromatographic performance.
General advice: Replace the guard column when the nature of the sample is changing, when backpressure is increased about 10-15%, or by schedule/time basis (for example, every 200 to 500 injections depending on the injection volume and sample purity).
Typical analytical guard columns are 1 or 2 cm long with either 2.0 (2.1) or 4.0 (4.6) mm depending on the column dimensions and 1cm long for 10mm, 21.2mm 30mm column id. Whether you opt for 1 or 2 cm long guard columns is tied to how harsh your mobile phase is and how messy your sample is.
AB: A LC column can be fine for a long period of time and has no defined shelf life, but it is dependent on what storage solvent was used for column storage. If the column was properly flushed after use and stored in 100% organic solvent such as acetonitrile, the column will survive for many years.
Below are some of the signs to look for that your relationship and your HPLC column lifetime is coming to an end. High Backpressure not reduced by reverse flushing the column. Split Peaks. Loss of resolution. Broad peak shape. Retention time shifts.
How can I prolong column lifetime? Sample preparation. Prefiltering samples prior to injection onto the column can help to limit the addition of particulates to the system. Mobile phase. The mobile phase is another factor that influences column lifetime. In-line filters and guard columns. Maintenance.
Below are some of the signs to look for that your relationship and your HPLC column lifetime is coming to an end. High Backpressure not reduced by reverse flushing the column. Split Peaks. Loss of resolution. Broad peak shape. Retention time shifts.

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