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[Music] the biorad gel excision kit comes with everything you need to safely excise agarose gel bands directly on the gel.go imaging system or other gel imagers to get started turn on your instrument pull out the transilluminator drawer and place desired tray on the trans illuminator wipe down the tray with alcohol to clear it of any particulates next carefully place your gel in the center of the tray with the ruler alongside it acquiring an image is quick and easy on the gel dot go system make sure to follow the instructions for your instrument when imaging the gel dot go blue tray can be used to locate bands without risking uv cross linking the ruler can be used as additional help to know you have selected the correct band for excision to excise your band grab a clean and unused plastic cutter use only one per band to eliminate cross contamination these plastic cutters will also reduce the chance of harming the glass tray or yourself [Music] throw away the used cutting tool another