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Welcome to the NDS Video Blog Series. In this video were diving into the DOT 5 Panel Drug Test - what substances are included and why its become the standard for urine-based drug testing. All DOT-regulated employers must perform DOT drug testing using the approved DOT 5 Panel Drug Test, with testing performed at a SAMHSA certified laboratory. As a DOT-regulated employer, youre required to drug test all DOT safety-sensitive covered employees. The most common examples are truck drivers, bus drivers, pilots, and railroad employees. You must follow the DOT regulations for drug testing, specifically 49 CFR Part 40. This regulation describes the required procedures for conducting workplace drug and alcohol testing for the Federally-regulated transportation industry. WHAT IS THE DOT 5 PANEL TEST? A common question we hear is, what is the DOT drug test? For many years, it was simply a 5 Panel drug test. And although there have been changes over the years, it is still called a 5 Panel drug t