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In this video tutorial, you will learn how to prepare uncontaminated bacterial culture using aseptic technique and investigate the effect of antibiotics on bacterial growth. Bacteria reproduce by binary fission and can double in number every 20 minutes with enough nutrients and suitable temperature. Bacterial culture can be done in a nutrient broth solution or agar gel plates. Nutrient broth provides all necessary nutrients for bacteria growth, while agar gel plates solidify the broth using agar, allowing for easy bacterial culturing. Triple biology students will benefit from understanding these concepts.
