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[Music] hi and welcome back to three sides I think a UK by the end of this video you should be able to describe how to prepare uncontaminated bacterial culture using aseptic technique you should then be able to describe how to investigate the effect of antibiotics on bacterial growth and both of these are triple biology students only in the last video we saw the bacteria reproduce by binary fission give enough nutrients in a suitable temperature bacteria can double in number every 20 minutes so in this video were looking how to coach a bacteria Im showing you a bacterial culture here now these bacteria are growing in a nutrient broth solution this contains all the nutrients that the bacteria need to grow and divide the broth cloudy because a contained a very large number of bacteria now another way to culture bacteria is to use an egg or gel plate which Im showing you here agar gel plates contain nutrient broth but thats been cycling to a jelly using a chemical called egg or this