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open imageJ open the interested image and then calibrate so first remove the existing scale by clicking click to remove scale and you can see pixels notice here the dimension of this image is 1280 by 1024 pixels It has not been calibrated yet, let us zoom into the scale bar this is 100 nm shift thats about right analyze set scale, 139 pixels, and we know its 100 nm okay so you can notice here the scale has been changed and for particle size analysis evenly illuminated image is very important next lets choose this area lets duplicate it now, lets do lets apply band-pass filter it will flatten the image to some extent and will reveal some details and you will be noticing some change and then we can threshold the image and we can see this is our original size of the nanoparticles and to what extent is okay to threshold, you can even zoom into the image and this looks, thats about right you can apply and then you can analyze particles in the beginning, you can just look at the outl
