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continuing on with our introduction to chromatography were going to be talking about separation and column resolution based on the number of equilibria that a mixture will undergo when we were talking in our last video we had been talking about relative retention alpha in terms of the bigger the difference between two partition ratios the better the separation if compounds have a large difference in partition ratios then theyll be easily separated with a minimum of number of equilibria but if you have two compounds that have very similar partition ratio is very similar affinities for the stationary phase its going to take a lot of equilibria to separate them therefore resolution or ability to separate things is dependent upon a ratio of their partition ratios and we call that alpha the relative retention and it is K Prime 2 thats the one that has the larger partition ratio divided by K Prime 1 so the ratio of those two partition ratios and it is always greater than 1 the bigger nu
