Correct stain in ppt smoothly

Aug 6th, 2022
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How to Correct stain in ppt

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the Consulting industry gets a lot of criticism for just making pretty slides and as a PowerPoint instructor for the Consulting industry I can tell you that theres definitely some validity to this but the truth is presentation does matter design formatting consistency structure all these things have an impact on your audiences perception of you your team and your organization and even more important than all of that the design of your presentation can impact your audiences ability to understand what it is youre trying to tell them which at the end of the day is your ultimate goal take this slide for example sure its painful to look at but the bigger problem is that its hard to understand I dont know where I should focus my attention I dont know what the slide is saying and I dont know why I should even care as frustrating as these slides can be they are fixable in another video I showed you how to build a slide from scratch but in this video Im going to show you what to do wi

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Simple staining involves directly staining the bacterial cell with a positively charged dye in order to see bacterial detail, in contrast to negative staining where the bacteria remain unstained against a dark background.
Gram Staining Instructions Heat fix the slide. Stain with Crystal Violet for 1 minute by flooding the slide with stain. Apply Iodine solution for 1 minute by flooding the slide with iodine. CAREFULLY, decolorize for 3 seconds with Gram Stain Decolorizer by flooding the slide with decolorizer.
The aim of simple staining is to study the morphology and arrangement of bacterial cells. The most commonly dyes used in simple staining are methylene blue, crystal violet and carbolfuchsin. Requirements. Cultures of Escherichia coli, Bacillus subtilis and Staphylococccusaureus. Staining solution of methylene blue.
Simple staining is a procedure where a single type of stain or dye is used to help visualize bacterial cell size, arrangement, and morphology, and make measurements, on the microscope. Bacterial cells appear transparent, but adding stains give color and contrast enabling one to observe the cells.
Negative staining requires the use of acid stain such as the Indian ink or nigrosin. The acid stain with its negatively charged chromogen will not penetrate the cells because of the negative charge on the surface of the bacteria.
Simple Staining 2. Differential Staining 3. Gram Staining 4. Acid Fast Staining 5.
Based on the nature of chromogen, there are three types of stain. Acidic stain (Anionic stain) Basic stain (Cationic stain) neutral stain.
1) Treatment with 0.5% Iodine solution. 2) Counter staining with safranin. 3) Treatment with absolute ethanol. 4) Staining heat fixed smear with crystal violet.
In simple staining, the bacterial cells are first fixed on a clean, oil-free slide and then flooded with stain (safranin, methylene blue, carbol fuchsin, Crystal violet, etc ). This stain will produce a distinctive contrast between the organism and its background so that we can easily distinguish them.
Simple staining is the procedure of using only one stain on the slide. This staining either colours the cells or colours the background. Hence, simple staining can be used to analyse only one organism per slide.

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