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[music] 50 x 2.1, 100 by 3, 150 by 4.6 which column is best for your application? HPLC column dimensions are an important selection during method development as they impact the efficiency, sensitivity, and speed of your analysis. You want to be sure that your choice in column dimension can accomplish both your chromatography and any additional application objectives, such as sensitivity, reduced solvent consumption, or instrument compatibility. It is important to consider both column length and inner diameter (ID) when choosing your column dimension. When considering your columns length, ask yourself how much resolution is actually needed. A longer column is not always better. Remember that efficiency is directly proportional to column length. This approach has its limitations as doubling column length increases resolution, but only by a factor of 1.4 while also docHubly increasing back pressure. Short column lengths, such as 50 mm, will give short run times and are ideal for g