Check columns resolution easily

Aug 6th, 2022
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How to check columns resolution

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continuing on with our introduction to chromatography were going to be talking about separation and column resolution based on the number of equilibria that a mixture will undergo when we were talking in our last video we had been talking about relative retention alpha in terms of the bigger the difference between two partition ratios the better the separation if compounds have a large difference in partition ratios then theyll be easily separated with a minimum of number of equilibria but if you have two compounds that have very similar partition ratio is very similar affinities for the stationary phase its going to take a lot of equilibria to separate them therefore resolution or ability to separate things is dependent upon a ratio of their partition ratios and we call that alpha the relative retention and it is K Prime 2 thats the one that has the larger partition ratio divided by K Prime 1 so the ratio of those two partition ratios and it is always greater than 1 the bigger nu

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On the first column click A to select the column, and then right click and select Column Width. Type the width that you want for your column. Click OK.
To resize a column to best fit its contents, position the pointer on the edge of the column that you want to resize. When the pointer becomes a double-headed arrow, double-click the edge of the column.
Freeze columns and rows Select the cell below the rows and to the right of the columns you want to keep visible when you scroll. Select View Freeze Panes Freeze Panes.
1. How to change cell size in Excel. Alternatively, to make all cells the same size, press Ctrl + A and drag rows and columns to a desired pixel size (as you drag and resize, Excel will display the row height and column width in points / units and pixels).
Best Fit Option To best fit one column, right click on the column header and select Best Fit. The selected column will adjust to display the full contents of the column. To best fit the entire gird, right click on any column header and select Best Fit (all columns).
Set a column to a specific width Select the column or columns that you want to change. On the Home tab, in the Cells group, click Format. Under Cell Size, click Column Width. In the Column width box, type the value that you want. Click OK.
Well, longer columns do provide higher plate counts (more plates per column) meaning that the elution bands are sharper (reduced fraction volume) which is what you want when performing analytical HPLC. Wider columns, however, provide increased loading capacity and this is what preparative chromatography needs.
It is a well-known fact that the separation efficiency of a chromatographic column increases with an increase in its length. Doubling the column length increases resolution by around 40%.
Chromatographic parameters The advantage of switching to longer columns is that the theoretical plates will increase and so will the resolution. The number of theoretical plates (N) is directly proportional to the length of the column and inversely proportional to the particle diameter2.
On the Excel menu, click Preferences. On the Ruler units menu, click the unit of measurement that you want to use.

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