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Hello everyone! Welcome to the microscopy lecture series. My name is Xiaowei Zhuang. And I am a professor at Harvard University and an investigator of Howard Hughes Medical Institute. And today what I am going to tell you about is a brief introduction of super-resolution fluorescence microscopy, which breaks the diffraction limit. And such imaging methods allow us to look at cells with much better clarity. For example, here we see a conventional image of mitochondria. And here is a three dimensional super-resolution image of mitochondria. So Ill tell you a few methods that allow us to get there. But first let me tell you why we like fluorescence microscopy so much. As we all know, this is one of the most widely used imaging modality for biological research. And two of the advantages of fluorescence microscopy is really very well recapitulated by this movie that I downloaded from the book . by Bruce Alberts et al. So first right away jumping into the scene even without even knowing w
