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Certify columns release

Certify columns release easily

Aug 6th, 2022

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How to certify columns release

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34 votes

In the video tutorial, parameters such as column size, type of resin, packing volume, and flow rate are crucial for successful operation. It is important to adhere to experimental requirements and resin instructions when estimating the amount of slurry needed based on the final column volume. Most resins are stored in a 75% slurry concentration in ethanol solution. To calculate the slurry volume needed for a certain column volume, use the equation provided. For example, for a 10 ml column with 75% slurry concentration and compression factor of 1.15, the required slurry volume is 15.3 ml. Ensure thorough washing of the resin before packing, using a fritted glass funnel.

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